Les principes fondamentaux de la préparation de l’ADN sont plus faciles à comprendre en examinant d’abord le type le plus simple de procédure de purification d’ADN, celui où le complément d’ADN entier d’une cellule bactérienne est nécessaire. Les modifications nécessaires pour la préparation d’ADN de plasmide et de phage peuvent ensuite être décrites ultérieurement. La […]
Posts from February 2017
Chapter 8: chemical kinetics – reactions in solution
The big difference between a solution and a gas is the presence of a solvent. A viscous solvent means that there are more collisions between the molecules of reactant and the molecules of solvent, slowing down the molecules. The first obligation to obtain a reaction is that the reactants have to be close to each […]
Chapter 8: chemical kinetics – kinetic theories
The following theories only apply to elementary reactions, i.e. in reactions in one step. The empirical relation of Arrhenius can be used (this relation is not limited to the elementary reactions): with kexp the kinetic constant found experimentally, Aexp the pre-exponential factor (same dimensions as kexp) and Ea the energy of activation. The goal is […]